期刊
EXPERIMENTAL NEUROLOGY
卷 218, 期 2, 页码 228-234出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.expneurol.2009.04.003
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- PRIN
- Ricerca Finalizzata
- CARIPE
Zn2+ can enter mitochondria and promote a plethora of physiological and patho-physiological effects. The issue of measuring changes in intramitochondrial levels is therefore critical. Past studies have employed fluorescent Zn2+ indicators, like Rhod-2 and RhodZin-3, however, the use of these probes is impaired by their extramitochondrial sequestration. In this study, we show that the ratiometric mitochondria-targeted pericam, RPmt, can be employed to detect changes of intramitochondrial free Zn2+ ([Zn2+](m)) levels. Using RPmt in neuronal and non neuronal cell lines we demonstrate that mitochondria can take Lip the cation mobilized from the cytosolic pool of protein-bound Zn2+ and that mitochondrial Zn2+ sequestration is largely mediated by the activity of the Ca2+ uniporter. (C) 2009 Elsevier Inc. All rights reserved.
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