期刊
EXPERIMENTAL HEMATOLOGY
卷 41, 期 5, 页码 479-490出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.exphem.2013.02.003
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资金
- National Institutes of Health (NTH) [P01 HL 32262, DK067356]
- NIH National Research Service Award Fellowship
- Diamond-Blackfan Anemia Foundation
- Swedish Research Council
- Maja och Hjalmar Leanders Stiftelse
- Sweden-America Foundation
We have developed a coculture system that establishes DLK+ fetal hepatic progenitors as the authentic supportive cells for expansion of hematopoietic stem (HSCs) and progenitor cells. In 1-week cultures supplemented with serum and supportive cytokines, both cocultured DLK+ fetal hepatic progenitors and their conditioned medium supported rapid expansion of hematopoietic progenitors and a small increase in HSC numbers. In 2- and 3-week cultures DLK+ cells, but not their conditioned medium, continuously and significantly (>20-fold) expanded both hematopoietic stem and progenitor cells. Physical contact between HSCs and DLK+ cells was crucial to maintaining this long-term expansion. Similar HSC expansion (approximately sevenfold) was achieved in cocultures using a serum-free, low cytokine- containing medium. In contrast, DLK- cells are incapable of expanding hematopoietic cells, demonstrating that hepatic progenitors are the principle supportive cells for HSC expansion in the fetal liver. (C) 2013 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.
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