期刊
EXPERIMENTAL HEMATOLOGY
卷 37, 期 1, 页码 101-110出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.exphem.2008.09.009
关键词
-
资金
- Center for Stem Cell and Regenerative Medicine [Tech 06-063]
- Third Frontier Program of Ohio
- NIH [5R01EB007350-02, R21HL072088]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R21HL072088] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB007350] Funding Source: NIH RePORTER
Objective. Methods producing human platelets using growth on plastic, on feeder layers, or in suspension have been described. We hypothesized that growth of hematopoietic progenitors in a three-dimensional (31)) scaffold would enhance platelet production sans feeder layer. Materials and Methods. We grew CD34 positively selected human cord blood cells in surgical-grade woven polyester fabric or purpose-built hydrogel scaffolds using it cocktail of cytokines. Results. We found production or functional platelets over 10 days with two-dimensional (2D), 24 days with 3D scaffolds in wells, and more than 32 days in a single-pass 3D perfusion bioreactor system. Platelet numbers produced daily were higher in 3D than 2D, and much higher in the 3D perfusion bioreactor system. Platelet output increased in hydrogel scaffolds coated with thrombopoietin and/or fibronectin, although this effect was largely obviated with markedly increased production caused by changes in added cytokines. In response to thrombin, the platelets produced aggregated and displayed increased surface CD62 and CD63. Conclusion. Use of 3D scaffolds, especially in a bioreactor-maintained milieu, may allow construction of devices for clinical platelet production without cellular feeder layers. (C) 2009 ISEH - Society for Hematology and Stern Cells. Published by Elsevier Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据