期刊
EXPERIMENTAL GERONTOLOGY
卷 46, 期 1, 页码 23-29出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exger.2010.09.011
关键词
Sarcopenia; Calorie restriction; Rhesus monkey
资金
- NIH [P01 AG-11915, P51 RR000167]
- Ellison Medical Foundation
- Research Facilities Improvement Program [RR15459-01, RR020141-01]
- NATIONAL CENTER FOR RESEARCH RESOURCES [P51RR000167, C06RR020141, C06RR015459] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R01AG040178, P01AG011915, R01AG037000] Funding Source: NIH RePORTER
Sarcopenia is the age-related loss of skeletal muscle mass and function and is characterized by a reduction in muscle mass and fiber cross-sectional area, alterations in muscle fiber type and mitochondrial functional changes. In rhesus monkeys, calorie restriction (CR) without malnutrition improves survival and delays the onset of age-associated diseases and disorders including sarcopenia. We present a longitudinal study on the impact of CR on early stage sarcopenia in the upper leg of monkeys from similar to 16 years to similar to 22 years of age. Using dual-energy X-ray absorptiometry we show that CR delayed the development of maximum muscle mass and, unlike Control animals, muscle mass of the upper leg was preserved in CR animals during early phase sarcopenia. Histochemical analyses of vastus lateralis muscle biopsies revealed that CR opposed age-related changes in the proportion of Type II muscle fibers and fiber cross-sectional area. In contrast the number of muscle fibers with mitochondrial electron transport system enzyme abnormalities (ETSab) was not significantly affected by CR. Laser capture microdissection of ETSab fibers and subsequent PCR analysis of the mitochondria! DNA revealed large deletion mutations in fibers with abnormal mitochondrial enzyme activities. CR did not prevent stochastic mitochondrial deletion mutations in muscle fibers but CR may have contributed to the maintenance of affected fibers. (c) 2010 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据