期刊
EXPERIMENTAL DERMATOLOGY
卷 19, 期 2, 页码 117-122出版社
WILEY
DOI: 10.1111/j.1600-0625.2009.00998.x
关键词
basal cell carcinoma; chemokine; Mast cell; PAR-2; psoriasis; tryptase
类别
资金
- Swedish Research Council - Medicine
- Swedish Cancer Foundation
- King Gustaf V 80-years foundation
- Ollie and Elof Ericsson's Foundation
- Ellen, Walter and Lennart Hesselmans Foundation
- Karolinska Institutet
- Kuopio University Hospital
- pharmaceutical company Abbott Finland
- European Union [504926]
Mast cells are increasingly present in the lesional skin of chronic skin inflammatory diseases including psoriasis and basal cell carcinoma (BCC). It has previously been shown that proteinase-activated receptor (PAR)-2 is expressed by mast cells, and tryptase is a potent activator of this receptor. In this study, skin biopsies from both healthy-looking and lesional skin of patients with psoriasis and superficial spreading BCC were collected and the expression of PAR-2 immunoreactivity in tryptase-positive mast cells was analysed. PAR-2 expression was confirmed in vitro in different mast cell populations. Cord-blood derived mast cells (CBMC) were stimulated with a PAR-2 activating peptide, 2-furoyl-LIGRLO-NH2. Consequently, IL-8 and histamine production was analysed in the supernatants. We observed a significant increase in the percentage of mast cells expressing PAR-2 in the lesional skin of psoriasis and BCC patients compared with the healthy-looking skin. HMC-1.2, LAD-2 and CBMC mast cells all expressed PAR-2 both intracellularly and on the cell surface. CBMC activation with the PAR-2 activating peptide resulted in an increased secretion of IL-8, but no histamine release was observed. Furthermore, both PAR-2 and IL-8 were co-localized to the same tryptase-positive mast cells in the lesional BCC skin. These results show that mast cells express increased levels of PAR-2 in chronic skin inflammation. Also, mast cells can be activated by a PAR-2 agonist to secrete IL-8, a chemokine which can contribute to the progress of inflammation.
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