期刊
EXPERIMENTAL CELL RESEARCH
卷 323, 期 1, 页码 100-111出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2014.02.025
关键词
GPNMB/osteoactivin; Prostate cancer; Invasivity; Apoptosis; Metalloproteinases
资金
- Fondazione Guido Berlucchi per la Ricerca sul cancro onlus (Borgonato, Bs, Italy)
Non-metastatic glycoprotein melanoma protein B (GPNMB), also known as osteoactivin (OA) is expressed in a wide array of tumors and represents an emerging target for drug development. In this study, we investigated the role of GPNMB/OA in the progression of human metastatic DU145 and PC3 prostate cancer cells. GPNMB/OA contribution in PCa malignant phenotype has been analyzed by small interfering RNA-induced GPNMB/OA silencing. We found that following GPNMB/OA silencing the migration capability of both DU145 and PC3 cells, evaluated by using in vitro invasivity assay, as well as the metalloproteinases MMP-2 and MMP-9 activity were equally strongly inhibited. By contrast knocking down GPNMB/OA weakly attenuated cell proliferation rate of DU145, an effect that paralleled with an increase number of apoptotic cells. However, PC3 cell growth seems to be not affected by GPNMB/OA. Together, these data reveal that GPNMB/OA acts as a critical molecular mediator promoting the acquisition of the more aggressive, pro-metastatic phenotype distinctive of human DU145 and PC3 cell lines. (C) 2014 Elsevier Inc. All rights reserved.
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