期刊
EXPERIMENTAL CELL RESEARCH
卷 316, 期 4, 页码 543-553出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2009.11.003
关键词
Molecular chaperone; Chaperonin; Cytoskeleton; Actin
资金
- Swedish Research Council
- Assar Gabrielsson's Fond
- Carl Tryggers Stiftelse
- Royal Society of Arts and Sciences in Goteborg
The integrity of the cytoskeleton is closely linked to the oligomeric chaperonin containing TCP-1 (CCT) via the folding requirements of actin and tubulin, but the role of CCT in cytoskeletal Organization remains unclear. We address this issue by analyzing the effects of targeting CCT subunits via siRNA and assessing their location/assembly state in cultured mammalian cells. Reducing levels of individual CCT subunits implicates CCT epsilon in influencing cell shape and reduced levels of this Subunit limit the cells' ability to recover from microfilament depolymerization. Conversely, cells displayed enhanced microtubule regrowth when CCT subunit levels were altered by siRNA. Some CCT subunits co-localize with F-actin, whilst all are predominantly monomeric in extracts enriched for the cytoskeleton. This provides compelling evidence that some CCT subunits as monomers can influence cytoskeletal organization/polymerization. Therefore the activity of CCT may well extend beyond the folding of newly synthesized polypeptides, representing a novel function for CCT Subunits distinct from their role in the CCT oligomer. (C) 2009 Elsevier Inc. All rights reserved.
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