期刊
EXPERIMENTAL CELL RESEARCH
卷 316, 期 11, 页码 1856-1870出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2010.02.027
关键词
Muscular dystrophy; Cytoskeleton; Z-disc; Sarcomere; Filamin C
资金
- UK Medical Research Council
- British Heart Foundation [FS/06/60]
- Medical Research Council [MC_U142684171, MC_U142684168, MC_U142684172, MC_U142684175] Funding Source: researchfish
- MRC [MC_U142684172, MC_U142684168, MC_U142684171, MC_U142684175] Funding Source: UKRI
The KY protein underlies a form of muscular dystrophy in the mouse but its role in muscle remains elusive. Immunodetection of endogenous KY protein in C2C12-derived myotubes and expression of a recombinant form in neonatal cardiomyocytes indicated that KY is a Z-band associated protein. Moreover, characterization of a KY interacting protein fragment led to the identification of kin1 (Immunoglobulin-like and fibronectin type 3 domain containing 1). Igfn1 is a transcriptionally complex locus encoding many protein variants. A yeast two-hybrid screen identified the Z-band protein filamin C (FLNC) as an interacting partner. Consistent with this, expression of an IGFN1 recombinant fragment showed that the three N-terminal globular domains, common to at least five IGFN1 variants, are sufficient to provide Z-band targeting. Taken together, the yeast two-hybrid, biochemical and immunofluorescence data support the notion that KY, IGFN1 and FLNC are part of a Z-band associated protein complex likely to provide structural support to the skeletal muscle sarcomere. (C) 2010 Elsevier Inc. All rights reserved.
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