期刊
EXPERIMENTAL CELL RESEARCH
卷 316, 期 6, 页码 1019-1027出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2010.01.002
关键词
Dental pulp cells; LPA; ROCK; Cell migration
资金
- National Natural Science Foundation of China [30973322]
- Research Fund for the Doctoral Program of Higher Education of China [20090181110089]
During the dental pulp repair process, dental pulp cells (DPCs) migrate to the site of injury and differentiate into odontoblasts or odontoblast-like cells. Although migration of DPCs is an important reparative process, the underlying mechanism remains unknown. The objective of this study was to determine the roles of lysophosphatidic acid (LPA) and the Rho-associated kinase (ROCK) pathway in the migration and morphology of dental pulp cells and alpha smooth muscle actin expression in vitro. We demonstrated that both LPA and ROCK inhibition enhanced cell motility and that their combined effects significantly increased migration rate. LPA induced fine cytoskeleton assembly and increased the level of alpha smooth muscle actin (alpha-SMA). ROCK inhibition by Y-27632 and ROCK(-1+2) small interfering RNA (siRNA) resulted in less actin cytoskeleton formation, a lower alpha-SMA level, a star-like cellular morphology and membrane ruffling. LPA and ROCK inhibition induced activation of another Rho GTPase, Rac, which may explain how LPA and ROCK inhibition increases cellmigration and lamellipodium formation. (C) 2010 Elsevier Inc. All rights reserved
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