期刊
EXPERIMENTAL CELL RESEARCH
卷 315, 期 8, 页码 1533-1547出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2008.12.001
关键词
CSL family; Fission yeast; Adhesion; Cell division; Nuclear division; High-frequency diploidization
资金
- Charles University [157/2005/B-BIO/PrF]
- Czech Science Foundation [204/03/H066]
- Czech Ministry of Education, Youth and Sports [MSM0021620858, LC07032]
The CSL (CBF1/RBP-J kappa/Suppressor of Hairless/LAG-1) family is comprised of transcription factors essential for metazoan development, mostly due to their involvement in the Notch receptor signaling pathway. Recently, we identified two novel classes of CSL genes in the genomes of several fungal species, organisms lacking the Notch pathway. in this study, we characterized experimentally cbf11(+) and cbf12(+), the two CSL genes of Schizosaccharomyces pombe, in order to elucidate the CSL function in fungi. We provide evidence Supporting their identity as genuine CSL genes. Both cbf11(+) and cbf12(+) are non-essential; they have distinct expression profiles and code for nuclear proteins with transcription activation potential. Significantly, we demonstrated that Cbf11 recognizes specifically the canonical CSL response element GTG(A)/(G)GAA in vitro. The deletion of cbf(11+) is associated with growth phenotypes and altered colony morphology. Furthermore, we found that Cbf11 and Cbf12 play opposite roles in cell adhesion, nuclear and cell division and their coordination. Disturbed balance of the two CSL Proteins leads to cell separation defects (sep phenotype), cut phenotype, and high-frequency diploidization in heterothallic strains, Our data show that CSL proteins operate in an organism predating the Notch pathway, which should be of relevance to the understanding of (Notch-independent) CSL functions in metazoans. (C) 2008 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据