期刊
EXPERIMENTAL CELL RESEARCH
卷 315, 期 6, 页码 1040-1052出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2008.12.004
关键词
Endocytosis; Transport; Actin; Microtubules; KIAA0674; FKBP15; FKBP133; WIP
资金
- Swedish Research Council
- Swedish Foundation for Strategic Research
- VINNOVA Sweden
- European Commission [LSHG-CT-2006-031220]
- Swedish Cancer Foundation
We have previously identified a new gene with sequence homology to the WASP-family of actin regulators denoted WAFL (WASP and FKBP-like). Here we report a possible biological function for WAFL, by demonstrating an association to early endosomes via its central coiled-coil domain, Further we show by functional and structural studies that WAFL is associated with both microtubules and the actin filament system, the two means of transport of early endosomes. In addition, WAFL interacts with WASP-interacting protein (WIP) and actin, thus linking WAFL to actin dynamics. The use of RNAi depletion of WAFL shows that WAFL-deficient cells display delayed transport of endosomal cargo. Our findings are compatible with a model whereby WAFL is involved in the transport of early endosomes at the level of transition between microfilament-based and microtubule-based movement. (C) 2008 Elsevier Inc. All rights reserved.
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