期刊
EXPERIMENTAL CELL RESEARCH
卷 315, 期 13, 页码 2322-2331出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2009.04.015
关键词
Baculovirus; Chromosomal integration; Embryonic stem cells; Gene delivery; Medaka; pluripotency
资金
- Biomedical Research Council of Singapore [R-05-1-21-19-404, R-08-1-21-19-585, SBIC-SSCC C-002-2007]
- Ministry of Education of Singapore [R-154-000-285-112]
- National University of Singapore [R-154-000-153-720]
- Center for Advanced Bionanosystems
- Nanyang Technological University
- Ministry of Education of Singapore, Academic Research Fund [Tier 2 T206B3110]
Viral vectors hold promise and challenges in gene therapy. Specifically, we have previously shown that baculoviral (BV) vectors have a high efficiency of gene delivery in human embryonic stem (ES) cells. Here we report the development of a complementary system to further our evaluation by utilizing the laboratory fish medaka that has ES cell lines and tools for experimental analyses in vitro and in vivo. We show that BV vectors can give rise to almost 100% of transient gene delivery in the medaka ES cell line MES1. BV-transduced MES1 cells reproducibly (at approximately 10(-5)) produce GFP-expressing colonies that, upon manual isolation, develop into stable clones during 300 days of culture. Surprisingly, BV transduction can also mediate efficient gene integration in the medaka genome, as fluorescent in situ hybridization revealed the presence of the BV-delivered gfp transgene in multiple locations in nuclei and on various chromosomes of metaphase spreads. We show that BV transduction does not compromise the genome stability and pluripotency of MES1 cells. We conclude that BV can efficiently mediate gene delivery and chromosomal integration in medaka ES cells. Therefore, medaka provides a powerful system for analyzing the potential of BV-mediated gene delivery in stem cells and gene therapy. (C) 2009 Elsevier Inc. All rights reserved.
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