期刊
EXPERIMENTAL CELL RESEARCH
卷 315, 期 7, 页码 1171-1180出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2009.01.001
关键词
Astakine; ATP synthase; Prokineticin; Receptor; Invertebrate; Hematopoiesis
资金
- Swedish Science Research Council
- Swedish research Council Formas
- Swedish Foundation for International Cooperation in Research and Higher Education to Kenneth Soderhall
We have recently isolated an invertebrate cytokine from a freshwater crayfish, which we named astakine 1. Interestingly this protein is expressed exclusively in hemocytes and hematopoietic tissue and is essential for the release of new hemocytes into the open circulatory system of these animals. This astakine has a prokineticin (PK) domain but lacks the N-terminal AVIT amino acids and hence receptor binding may differ from vertebrate PKs. Accordingly, here we report that a receptor for astakine 1 on hematopoietic tissue (Hpt) cells is identical to the beta-subunit of F(1)ATP synthase. In this study we have used several different methods to clearly demonstrate that ATP-synthase is located on the plasma membrane of a subpopulation of Hpt cells and there may function as a receptor for astakine, whereas mature blood cells (hemocytes) do not have any ATP synthase on the outside of their plasma membranes. Our results clearly show that ATP synthase R subunits are present on the cell surface of Hpt cells and highlight the need for more detailed studies on intracellular traffic connections between mitochondria and other membrane compartments. (C) 2009 Elsevier Inc. All rights reserved.
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