期刊
EXPERIMENTAL CELL RESEARCH
卷 314, 期 19, 页码 3581-3592出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2008.09.007
关键词
EGF; PDGF; Erk1/2; Cell proliferation; Brown adipocytes
资金
- Swedish Cancer Society
Stimulation by both adrenergic and non-adrenergic pathways can induce proliferation of brown pre-adipocytes. To understand the signalling pathways involved in non-adrenergic stimulation of cell proliferation, we examined Erk1/2 activation. In primary cultures of mouse brown preadipocytes, both EGF(epidermal growth factor) and PDGF(platelet-derived growth factor) induced Erk1/2 activation. EGF-stimulated Erk1/2 activation involved Src tyrosine kinases, but not PKC or PI3K, whereas in PDGF-induced Erk1/2 activation, PI3K, PKC (probably the atypical isoform) and Src were involved sequentially. Both EGF and PDGF induced PI3K-dependent Akt activation that was not involved in Erk1/2 activation. By comparing effects of signalling inhibitors (wortmannin, SH-6, TPA, Go6983, PP2, PD98059) on EGF- and PDGF-induced Erk1/2 activation and cell proliferation (H-3-thymidine incorporation), we conclude that while the signal transduction pathways initiated by these growth factors are clearly markedly different, their effects on cell proliferation can be fully explained through their stimulation of Erk1/2 activation; thus Erk1/2 is a common, essential step for stimulation of proliferation in these cells. (C) 2008 Elsevier Inc. All rights reserved
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