期刊
EXPERIMENTAL BIOLOGY AND MEDICINE
卷 239, 期 2, 页码 240-249出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/1535370213514329
关键词
Creatine kinase; hypovolemia; 8-isoprostane; phosphorylation potential
资金
- United States Department of Defense [W911NF0910086]
- National Institute of Neurological Disorders and Stroke [NS076865]
- Graduate School of Biomedical Sciences, University of North Texas Health Science Center
Tourniquet-imposed ischemia-reperfusion of extremities generates reactive oxygen and nitrogen species (RONS), which can disrupt intermediary metabolism and ATP production. This study tested the hypothesis that fluid resuscitation with pyruvate, a natural antioxidant and metabolic fuel, ameliorates the deleterious effects of ischemia-reperfusion on intermediary metabolism in skeletal muscle. Anesthetized male goats (similar to 25 kg) were bled to a mean arterial pressure of 48 +/- 1 mm Hg and then subjected to 90 min hindlimb ischemia with a tourniquet and femoral crossclamp, followed by 4-h reperfusion. Lactated Ringers (LR) or pyruvate Ringers (PR) was infused intravenous for 90 min, from 30 min ischemia to 30 min reperfusion, to deliver 0.05 mmol kg(-1) min(-1) lactate or pyruvate. Time controls (TC) underwent neither hemorrhage nor hindlimb ischemia. Lipid peroxidation product 8-isoprostane, RONS-sensitive aconitase and creatine kinase activities, antioxidant superoxide dismutase activity, and phosphocreatine phosphorylation potential ([PCr]/[{Cr}{P-i}]), an index of tissue energy state, were measured in reperfused gastrocnemius at 90 min resuscitation (n = 6 all groups) and 3.5 h post-resuscitation (n = 8 TC, 9 LR, 10 PR). PR more effectively than LR suppressed 8-isoprostane formation, prevented inactivation of aconitase and creatine kinase, doubled superoxide dismutase activity, and augmented [PCr]/([Cr][P-i]). Pyruvate-enriched Ringer's is metabolically superior to Ringer's lactate for fluid resuscitation of tour-niqueted muscle.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据