期刊
EXPERIMENTAL BIOLOGY AND MEDICINE
卷 239, 期 10, 页码 1340-1351出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/1535370214539225
关键词
Adipogenesis; mesenchymal stem cells; umbilical cord; peroxisome proliferator-activated receptor gamma; insulin signaling
资金
- U.S. Department of Agriculture-ARS-CRIS [6251-51000-005-00D]
- National Institutes for Health [R01-DK084225]
- NIH-CTSA program [UL1-TR-000039, KL2-TR-000063]
The umbilical cord (UC) matrix is a source of multipotent mesenchymal stem cells (MSCs) that have adipogenic potential and thus can be a model to study adipogenesis. However, existing variability in adipocytic differentiation outcomes may be due to discrepancies in methods utilized for adipogenic differentiation. Additionally, functional characterization of UCMSCs as adipocytes has not been described. We tested the potential of three well-established adipogenic cocktails containing IBMX, dexamethasone, and insulin (MDI) plus indomethacin (MDI-I) or rosiglitazone (MDI-R) to stimulate adipocyte differentiation in UCMSCs. MDI, MDI-I, and MDI-R treatment significantly increased peroxisome proliferator-activated receptor gamma (PPAR gamma) and CCAAT-enhancer binding protein alpha (C/EBP alpha) mRNA and induced lipid droplet formation. However, MDI-I had the greatest impact on mRNA expression of PPAR gamma, C/EBP alpha, FABP4, GPD1, PLIN1, PLIN2, and ADIPOQ and lipid accumulation, whereas MDI showed the least. Interestingly, there were no treatment group differences in the amount of PPAR gamma protein. However, MDI-I treated cells had significantly more C/EBP alpha protein compared to MDI or MDI-R, suggesting that indomethacin-dependent increased C/EBP alpha may contribute to the adipogenesis-inducing potency of MDI-I. Additionally, bone morphogenetic protein 4 (BMP4) treatment of UCMSCs did not enhance responsiveness to MDI-induced differentiation. Finally to characterize adipocyte function, differentiated UCMSCs were stimulated with insulin and downstream signaling was assessed. Differentiated UCMSCs were responsive to insulin at two weeks but showed decreased sensitivity by five weeks following differentiation, suggesting that long-term differentiation may induce insulin resistance. Together, these data indicate that UCMSCs undergo adipogenesis when differentiated in MDI, MDI-I, and MDI-R, however the presence of indomethacin greatly enhances their adipogenic potential beyond that of rosiglitazone. Furthermore, our results suggest that insulin signaling pathways of differentiated UCMSCs are functionally similar to adipocytes.
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