期刊
EXPERIMENTAL BIOLOGY AND MEDICINE
卷 238, 期 1, 页码 12-22出版社
SAGE PUBLICATIONS LTD
DOI: 10.1258/ebm.2012.012101
关键词
hyperbaric oxygen; decompression sickness; heat shock protein 70; antioxidation; antiapoptosis
资金
- Chinese National Natural Science Fund [81171873]
Decompression sickness (DOS) is a major concern in diving and space walk. Hyperbaric oxygen (HBO) preconditioning has been proved to enhance tolerance to DCS via nitric oxide. Heat-shock protein (HSP) 70 was also found to have protective effects against DOS. We hypothesized that the beneficial effects of HBO preconditioning on DOS was related to levels of elevated HSP70. HSPs (70, 27 and 90) expressed in tissues of spinal cord and lung in rats was detected at different time points following HBO exposure by Western blot. HSP27 and HSP90 showed a slight but not significant increase after HBO. HSP70 increased and reached highest at 18 h following exposure before decreasing. Then rats were exposed to HBO and subjected to simulated air dive and rapid decompression to induce DOS 18 h after HBO. The severity of DOS, along with levels of HSP70 expression, as well as the extent of oxidative and apoptotic parameters in the lung and spinal cord were compared among different groups of rats pretreated with HBO, HBO plus NG-nitro-L-arginine-methyl ester (L-NAME), HBO plus quercetin or normobaric air. HBO preconditioning significantly reduced the morbidity of DOS (from 663% to 36.7%), reduced levels of oxidation (malondialdehyde, 8-hydroxyguanine and hydrogen peroxide) and apoptosis (caspase-3 and -9 activities and the number of apoptotic cells). L-NAME or quercetin eliminated most of the beneficial effects of HBO on DOS, and counteracted the stimulation of HSP70 by HBO. Bubbles in pulmonary artery were detected using ultrasound imaging to observe the possible effect of HBO preconditioning on DOS bubble formation. The amounts of bubbles in rats pretreated with HBO or air showed no difference. These results suggest that HSP70 was involved in the beneficial effects of HBO on DOS in rats, suspected be by the antioxidation and antiapoptosis effects.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据