期刊
EXPERIMENTAL BIOLOGY AND MEDICINE
卷 236, 期 6, 页码 714-722出版社
SAGE PUBLICATIONS LTD
DOI: 10.1258/ebm.2011.010347
关键词
5-aza-cytidine; beta-catenin; DLD-1; DNA methylation; WNT signaling
资金
- University of Illinois at Urbana-Champaign
- NIH [CA135262]
Colorectal cancer (CRC) is a leading fatal carcinoma worldwide. Our goal was to investigate the effects of genistein on WNT signaling, which is involved in colon epithelial cell growth and apoptosis. Human colon cancer cell line DLD-1 was treated for four days with 75 mu mol/L genistein. Decreased nuclear beta-catenin and increased phospho-beta-catenin accumulation was detected, showing a change in WNT signaling. Reverse transcriptase-polymerase chain reaction analysis showed increased sFRP2 (a WNT pathway antagonist) mRNA expression following the genistein treatment. Methylation selective polymerase chain reaction showed decreased methylation in two CpG islands of the sFRP2 gene following genistein treatment, similar to the effect of 5-aza-cytidine, a demethylation agent. We observed reduced DLD-1 cell viability and increased apoptosis with genistein treatment. Genistein inhibits beta-catenin-mediated WNT signaling through increasing sFRP2 gene expression by demethylating its silenced promoter in colon cancer cell line DLD-1.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据