期刊
EXPERIMENTAL BIOLOGY AND MEDICINE
卷 235, 期 5, 页码 623-632出版社
SAGE PUBLICATIONS LTD
DOI: 10.1258/ebm.2009.009293
关键词
salvianolic acid B; hepatocytes; apoptosis; receptors; tumor necrosis factor; type I; mitochondria; high content screening
资金
- National Natural Science Foundation of China [30772869, 30472047]
- Major State Basic Research Development Program of China [2006CB504801]
- Program of Shanghai Subject Chief Scientist [08XD14041]
- E-institute [E03008]
- Innovative Research Team in Universities of Shanghai Municipal Education Commission
Salvianolic acid B (Sal B) is a water-soluble compound found in the traditional Chinese medicine, Radix Salviae miltiorrhizae, and has been widely used to treat a variety of diseases in Asian cultures. Sal B was shown to inhibit apoptosis in many cell types, but its effect on hepatocyte apoptosis is unknown. In this study, we attempt to show that Sal B attenuates hepatocyte apoptosis and hepatic injury induced by lipopolysaccharide and D-galactosamine in mice. Sal B also inhibits apoptosis that is induced by the death receptor in the HL-7702 hepatocyte cell line. Apoptosis in vitro is determined by flow cytometry, DNA electrophoresis and high content screening assay. The antiapoptotic effect is generated by reducing the expression of tumor necrosis factor a receptor type 1, balancing the expression of Bcl-2 family members, decreasing the release of cytochrome C from the mitochondria into the cytosol and inhibiting activated Caspase-3. These findings suggest that Sal B can effectively inhibit hepatocyte apoptosis as well as the underlying mechanisms related to regulating mediators in death receptor and mitochondria! pathways.
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