期刊
EXPERIMENTAL BIOLOGY AND MEDICINE
卷 234, 期 12, 页码 1493-1502出版社
SAGE PUBLICATIONS LTD
DOI: 10.3181/0903-RM-110
关键词
cerebral circulation; potassium channels; two-pore domain potassium channels
资金
- American Heart Association [RO1 NS-46666, PO1 NS-038660, RO1 HL-088435, 0665100Y]
TWIK-2, a member of the Two-Pore Domain K channel family, is expressed in a number of mammalian tissues including the vascular system. The function of TWIK-2 is not known. The purpose of this study was to clone the TWIK-2 channel from the rat middle cerebral artery, express it in CHO cells, and characterize the channel's electrical properties. In light of the fact that there are no specific TWIK-2 inhibitors or activators, a better characterization of the channel should enhance our understanding of its role in the vascular system. TWIK-2 was cloned from the rat middle cerebral artery and expressed with an N-terminal green fluorescence protein (GFP) in CHO cells. We report that rTWIK-2-GFP currents were relatively linear at physiological K+ concentrations but become slightly inwardly rectifying in symmetrical K+. rTWIK-2-GFP was insensitive to 10 mM TEA, 3 mM 4-aminopyridine, and 10 mu M glibenclamide. However, rTWIK-2-GFP was inhibited by Ba2+ with 50% of the current being blocked at 80 mu M. rTWIK-2-GFP activity was enhanced 60% by 100 mu M arachidonic acid. The electrophysiological characteristics of TWIK-2 indicate that it could serve an important role in ion homeostasis and regulation of the membrane potential in arteries and arterioles. Exp Biol Med 234:1493-1502, 2009
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