期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 26, 期 6, 页码 915-923出版社
SPRINGER
DOI: 10.1007/s13361-015-1133-y
关键词
Metabolomics; MALDI; Mass spectrometric imaging; Multivariate data analysis; Muscles
资金
- National Institute of Health [P30 AG028740]
- Clinical and Translational Science Institute (CTSI) [UL1 TR000064]
- Southeast Center for Integrated Metabolomics (SECIM, NIH) [U24 DK097209]
Skeletal muscles are composed of heterogeneous muscle fibers that have different physiological, morphological, biochemical, and histological characteristics. In this work, skeletal muscles extensor digitorum longus, soleus, and whole gastrocnemius were analyzed by matrix-assisted laser desorption/ionization mass spectrometry to characterize small molecule metabolites of oxidative and glycolytic muscle fiber types as well as to visualize biomarker localization. Multivariate data analysis such as principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to extract significant features. Different metabolic fingerprints were observed from oxidative and glycolytic fibers. Higher abundances of biomolecules such as antioxidant anserine as well as acylcarnitines were observed in the glycolytic fibers, whereas taurine and some nucleotides were found to be localized in the oxidative fibers.
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