期刊
EXPERIMENTAL AND MOLECULAR PATHOLOGY
卷 88, 期 2, 页码 326-329出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.yexmp.2009.12.007
关键词
Muscle degeneration; Real Time PCR; TBP; HRPT; Beta-actin; GAPDH
类别
资金
- Scientific and Technological Research Council of Turkey (TUBITAK) [SBAG 1055364]
The real time PCR technique requires the normalization of the gene of interest to reference genes that are accepted to be ubiquitously expressed. The choice of the reference gene(s) needs to be determined by researchers according to the particular tissue or model of interest. The best normalization gene is not easy to decide, particularly if the investigated tissue displays architectural changes and structural reorganization. We have investigated the expression of four housekeeping genes that are widely used for the normalization purposes (TATA binding protein, beta actin, hypoxanthine-guanine phosphorybosyl transferase and glyceraldehyde-3-phosphate dehydrogenase) in a skeletal muscle degeneration model applied by the release of the Achilles tendon which leads to a time-course degeneration of the soleus and gastrocnemius muscles. This study indicates that the TATA binding protein and the beta actin gene to be the least effected in the course of degeneration induced by tenotomy in rat soleus and gastrocnemius muscle. (C) 2009 Elsevier Inc. All rights reserved.
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