期刊
EXPERIMENTAL AND MOLECULAR PATHOLOGY
卷 88, 期 2, 页码 311-315出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.yexmp.2009.12.002
关键词
Dilated cardiomyopathy; Bone marrow stem cells; Left ventricle
类别
资金
- NSFC [30800481]
Background: Bone marrow mesenchymal stem cells (BMSCs) could differentiate into cardiomyocytes. In this study, we wished to determine whether we could improve ventricular function in a dilated cardiomyopathy (DCM) model in the rabbit by injecting stem cells directly into the ventricular wall. Methods and results: Japanese rabbits were treated with doxorubicin to induce dilated cardiomyopathy. BMSCs were isolated from isogenic adult rabbits and expanded ex vivo. DCM-model animals received an injection directly into the ventricular wall with either BMSCs or saline (sham-operated control; a third control group underwent no operation). Heart function in all three groups was evaluated by echocardiography and cardiac catheterization. Hearts were later excised for histological and immunostaining analysis. Left ventricular function was significantly better in BMSC-transplanted animals than in controls. Cellular staining of BrdU and N-cadherin, indicating the presence of bone-marrow-derived cells, was found in BMSC-transplanted animals but not in control and sham-operated animals. Conclusions: BMSC transplantation can improve left ventricular function in DCM. Direct injection into the ventricular wall is an effective approach for stem cell delivery. Crown Copyright (C) 2009 Published by Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据