期刊
EXPERIMENTAL AND MOLECULAR MEDICINE
卷 44, 期 2, 页码 167-175出版社
KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY
DOI: 10.3858/emm.2012.44.2.012
关键词
apoptosis; argpyrimidine; cataract; diabetes mellitus, type 2; epithelial cells; glycosylation end products, advanced; lens, crystalline; NF-kappa B
资金
- Korea Institute of Oriental Medicine (KIOM) [K10040, K11040]
- National Research Council of Science & Technology (NST), Republic of Korea [K11040] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The formation of advanced glycation end products (AGEs) has been considered to be a potential causative factor of injury to lens epithelial cells (LECs). Damage of LECs is believed to contribute to cataract formation. The purpose of this study was to investigate the cytotoxic effect of AGEs on LECs both in vitro and in vivo. We examined the accumulation of argpyrimidine, a methylglyoxal-derived AGE, and the expression of apoptosis-related molecules including nuclear factor-kappaB (NF-kappa B), Bax, and Bcl-2 in the human LEC line HLE-B3 and in cataractous lenses of Zucker diabetic fatty (ZDF) rats, an animal model of type 2 diabetes. In cataractous lenses from twenty-one-week-old ZDF rats, LEC apoptosis was markedly increased, and the accumulation of argpyrimidine as well as subsequent activation of NF-kappa B in LECs were significantly enhanced. The ratio of Bax to Bcl-2 protein levels was also increased. In addition, the accumulation of argpyrimidine triggered apoptosis in methyl-glyoxal-treated HLE-B3 cells. However, the presence of pyridoxamine (an AGEs inhibitor) and pyrrolidine dithiocarbamate (a NF-kappa B inhibitor) prevented apoptosis in HLE-B3 cells through the inhibition of argpyrimidine formation and the blockage of NF-kappa B nuclear translocalization, respectively. These results suggest that the cellular accumulation of argpyrimidine in LECs is NF-kappa B-dependent and pro-apoptotic.
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