期刊
EXPERIMENTAL AND MOLECULAR MEDICINE
卷 41, 期 9, 页码 629-637出版社
NATURE PUBLISHING GROUP
DOI: 10.3858/emm.2009.41.9.069
关键词
cyclooxygenase 2; lipopolysaccharides; NF-kappa B; nitric oxide synthase type II; reactive oxygen species; superoxide dismutase
资金
- Korean Science and Engineering Foundation
- Korean Ministry of Commerce, Industry and Energy
Inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) have been known to be involved in various pathophysiological processes such as inflammation. This study was performed to determine the regulatory function of superoxide dismutase (SOD) on the LPS-induced expression of iNOS, and COX-2 in RAW 264.7 cells. When a cell-permeable SOD, Tat-SOD, was added to the culture medium of RAW 264.7 cells, it rapidly entered the cells in a dose-dependent manner. Treatment of RAW 264.7 cells with Tat-SOD led to decrease in LPS-induced ROS generation. Pretreatment with Tat-SOD significantly inhibited LPS-induced expression of iNOS and NO production but had no effect on the expression of COX-2 and PGE(2) production in RAW 264.7 cells. Tat-SOD inhibited LPS-induced NF-kappa B DNA binding activity, I kappa B alpha degradation and activation of MAP kinases. These data suggest that SOD differentially regulate expression of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells.
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