期刊
ANNALS OF NEUROLOGY
卷 77, 期 6, 页码 1027-1049出版社
WILEY
DOI: 10.1002/ana.24399
关键词
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资金
- Netherlands Organization for Scientific Research (NWO)-ALW
- Netherlands Organization for Scientific Research (NWO)-MAGW
- Netherlands Organization for Scientific Research (NWO)-ZON-MW
- EU EUROHEADPAIN grant [602633]
- LUMC Fellowship
- Marie Curie Career Integration grant
- CURE SUDEP research award
- Center for Medical Systems Biology in the framework of the Netherlands Genomics Initiative
- Neuro-Basic, European Research Counsel (ERC)-Advanced
- Neuro-Basic, European Research Counsel (ERC)- ERC-POC
- NWO-VENI
- NWO-VIDI
- EUR-Fellowship
ObjectiveDisrupting thalamocortical activity patterns has proven to be a promising approach to stop generalized spike-and-wave discharges (GSWDs) characteristic of absence seizures. Here, we investigated to what extent modulation of neuronal firing in cerebellar nuclei (CN), which are anatomically in an advantageous position to disrupt cortical oscillations through their innervation of a wide variety of thalamic nuclei, is effective in controlling absence seizures. MethodsTwo unrelated mouse models of generalized absence seizures were used: the natural mutant tottering, which is characterized by a missense mutation in Cacna1a, and inbred C3H/HeOuJ. While simultaneously recording single CN neuron activity and electrocorticogram in awake animals, we investigated to what extent pharmacologically increased or decreased CN neuron activity could modulate GSWD occurrence as well as short-lasting, on-demand CN stimulation could disrupt epileptic seizures. ResultsWe found that a subset of CN neurons show phase-locked oscillatory firing during GSWDs and that manipulating this activity modulates GSWD occurrence. Inhibiting CN neuron action potential firing by local application of the -aminobutyric acid type A (GABA-A) agonist muscimol increased GSWD occurrence up to 37-fold, whereas increasing the frequency and regularity of CN neuron firing with the use of GABA-A antagonist gabazine decimated its occurrence. A single short-lasting (30-300 milliseconds) optogenetic stimulation of CN neuron activity abruptly stopped GSWDs, even when applied unilaterally. Using a closed-loop system, GSWDs were detected and stopped within 500 milliseconds. InterpretationCN neurons are potent modulators of pathological oscillations in thalamocortical network activity during absence seizures, and their potential therapeutic benefit for controlling other types of generalized epilepsies should be evaluated. Ann Neurol 2015;77:1027-1049
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