期刊
EUROPEAN RESPIRATORY JOURNAL
卷 32, 期 6, 页码 1529-1539出版社
EUROPEAN RESPIRATORY SOC JOURNALS LTD
DOI: 10.1183/09031936.00054608
关键词
Airway smooth muscle; BAPTA-AM; capacitative calcium entry; nonselective cation channel; single channel properties
资金
- Canadian Institutes of Health Research [MOP 15561]
- Ontario Thoracic Society
Passive depletion of internal Ca2+ stores in airway smooth muscle (ASM) activates nonselective cation channels (NSCCs) that mediate capacitative Ca2+ entry. However, the single channel properties of these cation channels have yet to be resolved and their regulation by cytosolilc Ca2+ levels ([Ca2+]i) still remains unclear. NSCC currents and changes in [Ca2+]i during passive depletion of internal Ca2+ stores were monitored in isolated bovine tracheal myocytes. Loading cells with 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetracetic acid acetyl methyl ester (BAPTA-AM) to reduce [Ca2+]i and thereby deplete the store augmented a basal Gd3+- and La3+-sensitive, Ca2+-permeable NSCC current. This current mimics that which is evoked by store depletion using the sarcoplasmic reticulum Ca2+ pump inhibitor cyclopiazonic acid (which concurrently and transiently elevates [Ca2+]i). Both interventions activated an similar to 25-pS NSCC with properties identical to both spontaneous (basal) and BAPTA-AM-evoked single channel currents. In summary, the present study provides novel evidence that a lanthanide-sensitive, 25-pS nonselective cation channel underlies both basal and store depletion-evoked membrane currents in airway smooth muscle and that this conductance likely contributes to the regulation of resting [Ca2+]i and capacitative Ca2+ entry.
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