期刊
EUROPEAN JOURNAL OF WILDLIFE RESEARCH
卷 55, 期 5, 页码 465-469出版社
SPRINGER
DOI: 10.1007/s10344-009-0262-7
关键词
PCV2; IHC; Molecular biology; Virus isolation; Electron microscopy
The lesions observed in 16 wild boars, hunted in central Italy, led to the suspect that could be related to the infection by porcine circovirus type 2 (PCV2). The animals had macroscopic and histological lesions in the lungs, tonsils, and bronchial lymph nodes. PCV2 was detected in tissue samples by polymerase chain reaction (PCR) and immunohistochemistry and it was isolated in newborn swine kidney cell cultures. From the infected cell culture supernatant, the presence of PCV2 DNA was confirmed by real-time PCR whereas virus particles were observed by electron microscopy. These diagnostic data indicate that PCV2 can infect and cause disease in Sus scrofa subspecies other than domestic swine and it is present in the wild boar population in central Italy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据