期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 137, 期 12, 页码 4066-4069出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.5b00944
关键词
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资金
- National Cancer Institute [5U54 CA151819]
- Ben and Catherine Ivy Foundation
- Jean Perkins Foundation
- Phelps Family Foundation
We describe chemical approaches for integrated metabolic and proteomic assays from single cells. Quantitative assays for intracellular metabolites, including glucose uptake and three other species, are designed as surface-competitive binding assays with fluorescence readouts. This enables integration into a microarray format with functional protein immunoassays, all of which are incorporated into the microchambers of a single-cell barcode chip (SCBC). By using the SCBC, we interrogate the response of human-derived glioblastoma cancer cells to epidermal growth factor receptor inhibition. We report, for the first time, on both the intercellular metabolic heterogeneity as well as the baseline and drug-induced changes in the metabolite-phosphoprotein correlation network.
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