期刊
EUROPEAN JOURNAL OF SOIL BIOLOGY
卷 62, 期 -, 页码 113-120出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.ejsobi.2014.03.005
关键词
Tea (Camellia sinensis); High temperature; Soil moisture; Microbial community; Functional genes
资金
- Research Fund for the Doctoral Program of Higher Education of China [20130101110127]
- Project of Zhejiang Key Scientific and Technological Innovation Team [2010R50039]
Tea (Camellia sinensis) is an important cash crop in China. However the response of the microbial communities in tea orchard soil to high temperature induced by climate change remains poorly understood. An incubation experiment where the soil was taken from a tea field, involving two temperatures (25 degrees C and 35 degrees C) and three levels of water saturation (30%, 55% and 80% water-filled pore space (WFPS)) was conducted. The abundance of the three microbial domains (bacteria, archaea and fungi) and functional genes related to the transformation of nitrogen were studied by real-time PCR. The response of microbial communities to different treatments were monitored by Terminal Restriction Fragment Length Polymorphism (T-RFLP) of PCR amplified rRNA gene sequences or internal transcribed spacer (ITS) sequence fragments. High temperature limited the transformation of ammonium to nitrate, increased the pH and repressed all analyzed genes except for the fungal marker genes (ITS) at 30% and 55% WFPS. However, at 80% WFPS, all genes at 35 degrees C, except bacterial amoA, increased and exceeded their abundance at 25 degrees C at the end of incubation. High temperature and soil moisture significantly influenced the abundance and diversity of all three domains because of a change in the relative abundance of dominant T-RFs or selection of new dominant T-RFs. The response of all three domains to temperature and different water regimes increased over time. Overall the study demonstrates the profound effects of an elevated temperature on the abundance of genes involved in the nitrogen cycle and the diversity of the dominant microbial community members. (C) 2014 Elsevier Masson SAS. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据