期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 137, 期 26, 页码 8521-8525出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.5b03413
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资金
- CNRS
- Universite de Bourgogne (uB)
- Conseil Regional de Bourgogne (CRB)
Visualization of DNA and RNA quadruplex formation in human cells was demonstrated recently with different quadruplex-specific antibodies. Despite the significant interest in these immunodetection approaches, dynamic detection of quadruplex in live cells remains elusive. Here, we report on NaphthoTASQ (N-TASQ), a next-generation quadruplex ligand that acts as a multiphoton turn-on fluorescent probe. Single-step incubation of human and mouse cells with N-TASQ enables the direct detection of RNA-quadruplexes in untreated cells (no fixation, permeabilization or mounting steps), thus offering a unique, unbiased visualization of quadruplexes in live cells.
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