期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 137, 期 15, 页码 4988-4992出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.5b01681
关键词
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资金
- Czech Science Foundation [P208/12/G016, P207/12/0775]
- Ministry of Education of the Czech Republic [LO1214, CZ.1.05/1.1.00/02.0123, LM2010005]
- Academy of Sciences of the Czech Republic
- Program of Employment of Best Young Scientists for International Cooperation Empowerment [CZ1.07/2.3.00/30.0037]
- European Social Fund
- state budget of the Czech Republic
- CERIT-SC under the program Center CERIT scientific Cloud [CZ.1.05/3.2.00/08.0144]
Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.
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