期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 137, 期 2, 页码 568-571出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja5107018
关键词
-
资金
- University of Geneva
- European Research Council (ERC)
- National Centre of Competence in Research (NCCR) Chemical Biology
- NCCR Molecular Systems Engineering
- Swiss NSF
In this report, fluorescent flippers are introduced to create planarizable pushpull probes with the mechanosensitivity and fluorescence lifetime needed for practical use in biology. Twisted pushpull scaffolds with large and bright dithienothiophenes and their S,S-dioxides as the first fluorescent flippers are shown to report on the lateral organization of lipid bilayers with quantum yields above 80% and lifetimes above 4 ns. Their planarization in liquid-ordered (Lo) and solid-ordered (So) membranes results in red shifts in excitation of up to +80 nm that can be transcribed into red shifts in emission of up to +140 nm by Forster resonance energy transfer (FRET). These unique properties are compatible with multidomain imaging in giant unilamellar vesicles (GUVs) and cells by confocal laser scanning or fluorescence lifetime imaging microscopy. Controls indicate that strong push-pull macrodipoles are important, operational probes do not relocate in response to lateral membrane reorganization, and two flippers are indeed needed to really swim, i.e., achieve high mechanosensitivity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据