期刊
EUROPEAN JOURNAL OF PLANT PATHOLOGY
卷 135, 期 4, 页码 903-911出版社
SPRINGER
DOI: 10.1007/s10658-012-0136-9
关键词
Fusarium oxysporum f. sp cubense race 4; Loop-mediated isothermal amplification (LAMP); PCR; Sequence-characterised amplified region (SCAR)
资金
- Science and Technology Innovation Foundation of FAAS [STIF-Y07]
- Commonweal Specialized Research Fund of China Agriculture [200903034]
- Doctor Foundation of FAAS
Fusarium wilt (Panama disease), caused by the fungus Fusarium oxysporum f. sp. cubense race 4 (Foc race 4), is one of the most destructive diseases affecting banana (Musa). Early and accurate detection of Foc race 4 is essential to protect the banana industry. We developed a novel and highly specific loop-mediated isothermal amplification (LAMP) assay for the detection of Foc race 4 based on a SCAR marker sequence. The detection limit for this assay was 10 fg per 25 mu l reaction in pure culture and DNA amplification was completed within 60 min. The assays detected 69 different isolates of Foc race 4 from geographically distinct counties in China, and no cross-reaction was observed with other fungal pathogens. When 26 infected and eight healthy looking but infested banana samples naturally from different fields were examined, the detection rate of LAMP was 100 %. The LAMP assay developed in this study was simple, fast, sensitive, and specific, and can be used in the field to detect Foc race 4 in infected banana plant tissue in resource-poor settings.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据