期刊
EUROPEAN JOURNAL OF PLANT PATHOLOGY
卷 125, 期 2, 页码 291-302出版社
SPRINGER
DOI: 10.1007/s10658-009-9482-7
关键词
Biotic stress; Combinatorial imaging; Fungal infection; Mildew disease; Pest management; Vitis vinifera
资金
- Academy of Sciences of the Czech Republic [AV0Z60870520, 2B06068, MSM6007665808, 522/09/1565]
- Ministry of Education, Youth and Sports of the Czech Republic
- Regional Administration of Friuli Venezia Giulia ( Italy)
Plasmopara viticola is an economically important pathogen of grapevine. Early detection of P. viticola infection can lead to improved fungicide treatment. Our study aimed to determine whether chlorophyll fluorescence (Chl-F) imaging can be used to reveal early stages of P. viticola infection under conditions similar to those occurring in commercial vineyards. Maximum (F-V/F-M) and effective quantum yield of photosystem II (I broken vertical bar(PSII)) were identified as the most sensitive reporters of the infection. Heterogeneous distribution of F-V/F-M and I broken vertical bar(PSII) in artificially inoculated leaves was associated with the presence of the developing mycelium 3 days before the occurrence of visible symptoms and 5 days before the release of spores. Significant changes of F-V/F-M and I broken vertical bar(PSII) were spatially coincident with localised spots of inoculation across the leaf lamina. Reduction of F-V/F-M was restricted to the leaf area that later yielded sporulation, while the area with significantly lower I broken vertical bar(PSII) was larger and probably reflected the leaf parts in which photosynthesis was impaired. Our results indicate that Chl-F can be used for the early detection of P. viticola infection. Because P. viticola does not expand systemically in the host tissues and the effects of infection are localised, Chl-F imaging at high resolution is necessary to reveal the disease in the field.
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