期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 730, 期 -, 页码 164-170出版社
ELSEVIER
DOI: 10.1016/j.ejphar.2014.02.025
关键词
Kir2.1; Inward rectify; Barium; Cardiac action potential; Outward current
资金
- Grants from Scientific Research & Development Fund of Zhejiang A F University [2013FR032]
Kir2.1 channel is a typical inward rectified channel with little outward currents when the membrane depolarized. Barium blocks the inward Kir2.1 currents in a voltage-dependent manner. However, in this study we found that barium would impair the rectification and open Kir2.1 outward currents at a depolarized voltage, causing increment of outward current amplitudes by 43 +/- 7% (n=5, P<0.01) after 200 s barium application. In the meanwhile, a higher barium concentration did block the outward currents by 17.5 +/- 4.3% (n=4, P<0.01) and temporarily twisted current upward tendency. The increment was likely barium specific since both calcium and Kir2.1 specific blocker, Chloroethylclonidine (CEC), did not enhance the current amplitudes. The rectification of Kir2.1 was not recovered by washing barium off, which suggested a non-competitive mechanism. Since the currents occurred at phase 1, 2 of cardiac action potential, it would likely shorten the action potential plateau and it would decrease QT duration in electrocardiography (ECG). (C) 2014 Elsevier B.V. All rights reserved.
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