期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 697, 期 1-3, 页码 32-39出版社
ELSEVIER
DOI: 10.1016/j.ejphar.2012.10.008
关键词
Bone marrow derived-neural stem cells; Salvianolic acid B; Self-renewal; Differentiation; Brain-derived neurotrophic factor (BDNF)
资金
- National Natural Science Foundation of China [81173580]
- Natural Science Foundation of Liaoning Province [201102144]
- Science Foundation of Shenyang City [F11-264-1-42]
Our previous study has demonstrated the therapeutic potential of bone marrow derived-neural stem cells (BM-NSCs) in CNS disorders; however, the beneficial effects are modest due to the poor survival and low neural differentiation frequency. Here, we demonstrate that salvianolic acid B (Sal B), a potent aqueous of a well known Chinese medicine herb, Salvia miltiorrhiza, possesses the ability to promote BM-NSCs proliferation in a dose dependent manner as verified by growth curve and Bromodeoxyuridine (BrdU) incorporation assays; While in differentiation medium, Sal B promoted nestin(+) BM-NSCs differentiated into greater numbers of NF-M+ neurons and NG2(+) oligodendrocyte precursors, but fewer GFAP(+) astrocytes as verified by triple immunostaining and quantitative analysis; upon exposure to H2O2, Sal B facilitated the cells survival, reduced LDH leakage, and inhibited apoptosis, displaying a dose-dependent neuroprotective effect on BM-NSCs. Sal B induced brain-derived neurotrophic factor (BDNF) production by BM-NSCs, which may be beneficial for the cells survival and differentiation in unfavourable environment. The collective evidence indicates that Sal B may be a potential drug to upgrade the therapeutic efficiency of BM-NSCs in CNS diseases. (C) 2012 Elsevier B.V. All rights reserved.
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