期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 643, 期 2-3, 页码 211-217出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2010.06.027
关键词
Shikonin; Cerebral ischemia/reperfusion; Antioxidant; Neuroprotection
资金
- National Natural Science Foundation of China [30760058]
- Xin Jiang Production and Construction Corps [ZD2007JC04, 2007GG23]
- Program for New Century Excellent Talents in University [NCET-06-0918]
The aim of our study was to investigate the neuroprotective properties of shikonin, a naphthoquinone pigment isolated from the roots of the traditional Chinese herb Lithospermum erythrorhizon. In the present study, mice were divided randomly into sham, model, shikonin and edaravone-treated groups. Shikonin (50, 25, and 12.5 mg/kg, i.g.) or maize oil was administered three times before ischemia and once at 2 h after the onset of ischemia. Mice were anesthetized with chloral hydrate and subjected to middle cerebral artery 2 h of occlusion and then 22 h of reperfusion. Different antioxidant assays were employed in order to evaluate the antioxidant activities of shikonin in vitro. Neurological deficit, infarct size, histopathology changes and oxidative stress markers were evaluated after 22 h of reperfusion. In comparison with the model group, treatment with shikonin significantly decreased neurological deficit scores, infarct size, the levels of malondialdehyde(MDA), carbonyl and reactive oxygen species, and attenuated neuronal damage, up-regulated superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) activities and reduced glutathione (GSH)/glutathione disulfide (GSSG) ratio. Taken together, these results suggested that the neuroprotective effects of shikonin against cerebral ischemia/reperfusion injury may be attributed to its antioxidant effects. (C) 2010 Elsevier B.V. All rights reserved.
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