期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 589, 期 1-3, 页码 127-131出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2008.04.063
关键词
gambogic acid; integrin; adhesion; metastasis; B16-F10 cell
资金
- National Natural Science Foundation of China [30472044, 30701032, 90713038]
- Natural Science Foundation of Jiangsu province (No. BK20055096 [BK20055096]
Gambogic acid, the major active ingredient of gamboge, has been shown to exhibit anti-cancer activity both in vivo and in vitro. However, its potential activity in tumor metastasis remains unclear. In the present study, we found that Gambogic acid strongly inhibited the adhesion of highly metastatic mouse melanoma B16-F10 cells in vitro. Gambogic acid also exhibited significant anti-metastasis activity on the development of in vivo artificial metastases (i.e. following tail vein i.v. injection of the B16-F10 melanoma tumor cells in C57BL/6 mice). Flow cytometric analysis and Western blot showed that Gambogic acid inhibited the cell surface expression of alpha(4) integrin. while exhibited negligible effects on the expression of alpha(5) and beta(1) integrin. Thus we concluded for the first time that Gambogic acid could inhibit the adhesion and migration of B16-F10 cells in vitro and in vivo, in which down-regulation of alpha(4) integrin expression was involved. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据