期刊
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
卷 79, 期 2, 页码 416-422出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejpb.2011.04.010
关键词
Passive drug permeability; Micelle; Solubilisation; Pluronic; Ketoprofen; Nadolol; Trans-epithelial electrical resistance
资金
- Phospholipid Research Center, Heidelberg, Germany
Drug permeability of the model drugs ketoprofen and nadolol across Caco-2 cell monolayers was determined in the absence and presence of the non-ionic surfactant Poloxamer 188 (Pluronic (R) F68, P-188). Stringent controls confirmed that P-188 in concentrations up to 50 mg/ml did not adversely affect cell viability or monolayer integrity. Equilibrium experiments confirmed that the drugs were merely passively transported. Caco-2 permeability of both drugs was found to be decreased by the surfactant in a concentration-dependent manner. Ultrafiltration revealed that both drugs were associated with surfactant micelles. The systematic investigation of micellization on passive absorption showed that association of drugs with P-188 micelles appears to depress their passive permeability under conditions here other transport mechanisms can be neglected. (C) 2011 Elsevier B.V. All rights reserved.
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