期刊
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
卷 49, 期 3, 页码 371-381出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejps.2013.03.011
关键词
Photodynamic therapy; Polymeric nanoparticles; Chloroaluminium phthalocyanine; Photophysical characterisation; Release kinetic; Phototoxicity in vitro
资金
- CNPq [481195/2011-4]
- FAPESP [2006/50562-1, 2009/15363-9, 2007/55319-0]
- NANOBIOMG/FAPEMIG Network, Minas Gerais, Brazil
- UFOP
- CAPES
Nanoparticles of poly(D,L-lactide-co-glycolide), poly(D,L-lactide) and polyethylene glycol-block-poly(D,L-lactide) were developed to encapsulate chloroaluminium phthalocyanine (AlClPc), a new hydrophobic photosensitiser used in photodynamic therapy (PDT). The mean nanoparticle size varied from 115 to 274 nm, and the encapsulation efficiency ranged from 57% to 96% due to drug precipitation induced by different types of polymer. All nanoparticle formulations presented negative zeta potential values (-37 mV to -59 mV), explaining their colloidal stability. The characteristic photophysical parameters were analysed: the absorption spectrum profile, fluorescence quantum yield and transient absorbance decay, with similar values for free and nanoparticles of AlClPc. The time-resolved spectroscopy measurements for AlClPc triplet excited state lifetimes indicate that encapsulation in nanocapsules increases triplet lifetime, which is advantageous for PDT efficiency. A sustained release profile over 168 h was obtained using external sink method. An in vitro phototoxic effect higher than 80% was observed in human fibroblasts at low laser light doses (3 J/cm(2)) with 10 mu M of AlClPc. The AlClPc loaded within polymeric nanocapsules presented suitable physical stability, improved photophysical properties, sustained released profile and suitable activity in vitro to be considered a promising formulation for PDT. (c) 2013 Elsevier B.V. All rights reserved.
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