期刊
EUROPEAN JOURNAL OF PAIN
卷 12, 期 8, 页码 1031-1039出版社
WILEY
DOI: 10.1016/j.ejpain.2008.01.011
关键词
Nerve injury; Dorsal rhizotomy; EphrinB/EphB receptor; Dorsal root ganglion; Spinal cord
资金
- Parker Research Foundation [PCCRF-BSR0501, PCCRF-BSR0602]
- National National Science Foundation of China [NSFC-306280217]
EphrinB-EphB receptor signaling plays diverse roles during, development, but recently has been implicated in synaptic plasticity in the matured nervous system and in pain processes. The present study investigated the correlation between expression of ephrinB and EphB receptor proteins and chronic constriction injury (CCI) of the sciatic nerve and dorsal rhizotomy (DR) in dorsal root ganglion (DRG) and spinal cord (SC) and interaction of CCI and DR on expression of these signals. Adult, male Sprague-Dawley rats were employed and thermal sensitivity was determined in the sham operated CCI and DR rats. Western blot and immunobiochemistry analysis and immunofluorescence staining techniques were used to detect the expression and location of the ephrinB-EphB receptor proteins in DRG and SC. The results showed that expression of ephrinB1 and EphB1 receptor proteins was significantly upregulated in DRG and SC in a time-dependent manner corresponding to the development of thermal hyperalgesia after CCI. The increased expression is predominately located in the medium and small-sized DRG neurons, the superficial layers of spinal dorsal horn (DH) neurons and the IB4 positive nociceptive terminals. DR increases ephrinB1 in DRG, not SC and EphB receptor in SC, not DRG. DR suppressed CCI-induced upregulation of ephrinB1 in SC and EphB1 receptor in DRG and SC. These findings indicate that ephrinB-EphB receptor activation and redistribution in DRG and DH neurons after nerve injury could contribute to neuropathic pain. This study may also provide a new mechanism underlying DR-induced analgesia in clinic. (C) 2008 European Federation of Chapters of the International Association for the Study of Pain. Published by Elsevier Ltd. All rights reserved.
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