期刊
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
卷 2009, 期 3, 页码 349-357出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ejoc.200800670
关键词
Solid-phase synthesis; Glycoconjugates; Carbohydrates; Proteins; Carbohydrate protein interactions; Carbamate linker; Gel-phase F-19 NMR spectroscopy; Microtiter plates
资金
- Swedish Research Council
- Kempe Foundation
Amino-functionalized serine-based galactose and glucose neoglycolipids were prepared by solid-phase synthesis using a carbamate strategy for anchoring amino functionalities to a (2-fluoro-4-hydroxymethylphenoxy)acetic acid linker resin. Key synthetic steps were monitored with gel-phase F-19 NMR spectroscopy. Cleavage from the solid support was performed with trifluoroacetic acid. The terminal amine of the neoglycolipids was conjugated with didecyl squarate and then immobilized in amino-functionalized microtiter plates and the glycoconjugates were successfully probed with a galactose-binding lectin. ((C) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
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