期刊
EUROPEAN JOURNAL OF ORAL SCIENCES
卷 119, 期 -, 页码 149-157出版社
WILEY
DOI: 10.1111/j.1600-0722.2011.00881.x
关键词
amelogenesis; early maturation; enamel organ; gene expression; late maturation
资金
- NIDCR NIH HHS [R01 DE019629, DE019629, R01 DE013404-09, R01 DE013404, DE013404, R01 DE019629-03] Funding Source: Medline
- NIGMS NIH HHS [R01 GM072447, R01 GM072477, GM072447, R01 GM072477-05S1] Funding Source: Medline
Enamel maturation is a dynamic process that involves high rates of mineral acquisition, associated fluctuations in extracellular pH, and resorption of extracellular enamel proteins. During maturation, ameloblasts change from having a tall, thin, and highly polarized organization, characteristic of the secretory stage, to having a low columnar and widened morphology in the maturation stage. To identify potential differences in gene expression throughout maturation, we obtained enamel organ epithelial cells derived from the early-and late-maturation stages of rat incisor and analyzed the global gene-expression profiles at each stage. Sixty-three candidate genes were identified as having potential roles in the maturation process. Quantitative PCR was used to confirm the results of this genome-wide analysis in a subset of genes. Transcripts enriched during late maturation (n = 38) included those associated with lysosomal activity, solute carrier transport, and calcium signaling. Also up-regulated were transcripts involved in cellular responses to oxidative stress, proton transport, cell death, and the immune system. Transcripts down-regulated during the late maturation stage (n = 25) included those with functions related to cell adhesion, cell signaling, and T-cell activation. These results indicate that ameloblasts undergo widespread molecular changes during the maturation stage of amelogenesis and hence provide a basis for future functional investigations into the mechanistic basis of enamel mineralization.
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