期刊
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY
卷 169, 期 1, 页码 28-32出版社
ELSEVIER
DOI: 10.1016/j.ejogrb.2013.01.025
关键词
microRNA; miRNA-17; miRNA-19b; Early pregnancy; Villous sample; PTEN; TGF-beta 1; CREB-1; TGF beta-RII
资金
- Grants-in-Aid for Scientific Research [24659737] Funding Source: KAKEN
Objective: First, to determine if microRNA-17 and -19b are expressed in villous samples at early stages of pregnancy. Second, to determine whether placental expressions of these microRNAs along with their main targets (PTEN, CREB-1, TGF beta-1 and TGF beta-RII) are altered in early pregnancy loss. Study design: Expression levels of microRNAs and mRNA targets in villous samples from early pregnancy loss (n = 11) and matched normal cases (n = 20) by gestational age were determined by RT-PCR. Results: Both microRNA-17 and -19b were expressed in all cases of normal pregnancy. They were significantly down-regulated (relative ratios: 0.35 and 0.34 respectively) in early pregnancy loss. Their main target, PTEN mRNA, was significantly up-regulated in early pregnancy loss (relative ratio: 2.6, 95%CI: 0.2-29.8). TGF-beta 1, CREB-1 and TGF beta-RII were not significantly different between the two groups. Conclusion: microRNA-17 and -19b are expressed in early stages of pregnancy. They are down-regulated in villous samples from early pregnancy loss. We suggest that these main members of the microRNA-17-92 cluster might be involved in placental invasion and its dysregulation might also be related to other conditions characterized by defective placentation. (c) 2013 Elsevier Ireland Ltd. All rights reserved.
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