期刊
EUROPEAN JOURNAL OF NEUROSCIENCE
卷 35, 期 4, 页码 527-538出版社
WILEY
DOI: 10.1111/j.1460-9568.2012.07992.x
关键词
conditional Vglut2 KO; diffuse transmission; electron microscopy; neostriatum; nucleus accumbens; tyrosine hydroxylase immunocytochemistry
资金
- Fonds de la Recherche en Sante du Quebec (FRSQ)
- CIHR [MOP-3544, MOP-106562, MOP-49951, MOP-106556]
- NARSAD
- FRSQ
- GRSNC (Groupe de Recherche sur le Systeme Nerveux Central)
- Swedish Foundation for International Cooperation in Research and Higher Education
- Swedish Research Council
- Swedish Brain Foundation
- Swedish Brain Power
- Ahlen and Wiberg Foundations
- National Board of Health and Welfare
- Uppsala University
Despite the increasing use of genetically modified mice to investigate the dopamine (DA) system, little is known about the ultrastructural features of the striatal DA innervation in the mouse. This issue is particularly relevant in view of recent evidence for expression of the vesicular glutamate transporter 2 (VGLUT2) by a subset of mesencephalic DA neurons in mouse as well as rat. We used immuno-electron microscopy to characterize tyrosine hydroxylase (TH)-labeled terminals in the core and shell of nucleus accumbens and the neostriatum of two mouse lines in which the Vglut2 gene was selectively disrupted in DA neurons (cKO), their control littermates, and C57BL/6/J wild-type mice, aged P15 or adult. The three regions were also examined in cKO mice and their controls of both ages after dual THVGLUT2 immunolabeling. Irrespective of the region, age and genotype, the TH-immunoreactive varicosities appeared similar in size, vesicular content, percentage with mitochondria, and exceedingly low frequency of synaptic membrane specialization. No dually labeled axon terminals were found at either age in control or in cKO mice. Unless TH and VGLUT2 are segregated in different axon terminals of the same neurons, these results favor the view that the glutamatergic cophenotype of mesencephalic DA neurons is more important during the early development of these neurons than for the establishment of their scarce synaptic connectivity. They also suggest that, in mouse even more than rat, the mesostriatal DA system operates mainly through non-targeted release of DA, diffuse transmission and the maintenance of an ambient DA level.
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