期刊
EUROPEAN JOURNAL OF NEUROLOGY
卷 21, 期 8, 页码 1096-1101出版社
WILEY
DOI: 10.1111/ene.12435
关键词
antibodies; demyelinating diseases; multiple sclerosis; neuroimmunology
资金
- Instituto de Salud Carlos III (Spain) [PI12/02349, PI12/00239]
- Ministerio de Economia y Competitividad (Spain) [SAF2012-34670]
- Instituto de Salud Carlos III-Fondo Europeo de Desarrollo Regional (Feder) [CP07/00273]
Background and purpose: Different data show an association between human herpesvirus 6 (HHV-6) and multiple sclerosis (MS). Intrathecal anti-HHV-6 immunoglobulin G (IgG) was detected in MS patients, but the antigen recognized by cerebrospinal fluid (CSF) IgG has not been characterized yet. Our objective was to identify the HHV-6 antigens recognized by IgG present in the CSF of patients with MS. Methods: Cerebrospinal fluid IgG of 15 MS patients and eight patients with other neurological diseases was purified on protein G Sepharose columns. Purified IgG from every patient was linked to a CNBr-activated Sepharose 4B column. Fifty micrograms of viral extract was applied to each column. Bound proteins were eluted and analysed by SDS-PAGE and silver staining. The viral protein was characterized by mass spectrometry. Results: A protein of 150 kD was eluted from CSF IgG columns of three of eight patients with primary progressive MS and one of seven with relapsing-remitting MS. After digestion and mass spectrometry analysis 10 peptides were found with 100% homology with the major capsid protein of the HHV-6A. Discussion: These findings confirm the presence of anti-HHV-6 IgG in CSF of MS patients, particularly in progressive forms, and identify major capsid protein as the major antigen recognized by CSF IgG from MS patients.
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