期刊
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
卷 66, 期 -, 页码 380-387出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.ejmech.2013.05.042
关键词
Oridonin; Resonance light scattering; DNA; Intracellular target; Binding mode
资金
- Natural Science Foundation of Guangdong Province [9151022501000019]
In this study, a sensitive and reliable assay has been developed to determine the binding mode of oridonin to DNA in vitro using resonance light scattering (RLS) technique. The binding mode of ORI with DNA has been confirmed by measuring the changes of RLS intensity under different pH and ionic strength. It is the ORI that is intercalated into the double helix DNA to destroy its template function and inhibit the synthesis of DNA. Moreover, the RLS assay result was validated by seasoned vitro methods. The proposed RLS assay is not only an unprecedented one for primarily determine the binding mode of oridonin to DNA, but also a simple, sensitive, objective and straightforward method for the detection of ORI. (C) 2013 Elsevier Masson SAS. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据