期刊
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
卷 44, 期 10, 页码 4162-4168出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.ejmech.2009.05.007
关键词
Oxidative stress; Antioxidant capacity; Chromium peroxide; Spectrophotometry; alpha-Tocopherol; Ascorbate
Oxidative stress plays a pivotal role in the ageing process and in the pathogenesis of numerable diseases. The quantification of the phenomenon is of paramount importance. in the present study, we introduce a novel and simple assay, the Blue CrO5 assay, for the evaluation of the oxidant and antioxidant capacity of various biological samples and known antioxidants. Chromium peroxide (CrO5) is produced by ammonium dichromate in an acidic environment in the presence of H2O2. it is a deep blue potent oxidant compound, miscible and relatively stable in polar organic solvents, that can be easily measured by spectrophotometry. Its reduction by known antioxidants, both water- and lipid-soluble (ascorbate and alpha-tocopherol, respectively, in this study), detected spectrophotometrically as a decrease in the absorption and depicted in EPR spectra, can act as a measure of the antioxidant capacity of a certain compound. The assay displays significant sensitivity, stability, linearity, specificity and repeatability. (C) 2009 Elsevier Masson SAS. All rights reserved.
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