期刊
EUROPEAN JOURNAL OF MASS SPECTROMETRY
卷 17, 期 4, 页码 333-343出版社
IM PUBLICATIONS
DOI: 10.1255/ejms.1140
关键词
electrospray ionization; additive; acidic proteins; RNA; DNA; negative ions
资金
- Austrian Science Fund (FWF) [Y372, 1317]
- Austrian Science Fund (FWF) [Y 372] Funding Source: researchfish
Acidic proteins and nucleic acids such as RNA are most readily ionized in electrospray ionization (ESI) operated in negative-ion mode. The multiply deprotonated protein or RNA ions can be used as precursors in top-down mass spectrometry. Because the performance of the dissociation method used critically depends on precursor ion negative net charge, it is important that the extent of charging in ESI can be manipulated efficiently. We show here that (M - nH)(n-) ion net charge of proteins and RNA can be controlled efficiently by the addition of organic bases to the electrosprayed solution. Our study also highlights the fact that ion formation in ESI in negative mode is only poorly understood.
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